Title应用聚合酶链反应检测食管癌组织中人乳头瘤病毒
Other TitlesDetection of human papillomavirus in tissues of esophageal carcinomas by polymerase chain reaction
Authors李淑英
李颖
王立东
吴晓舟
周玲
赵晓瑜
刘宏图
曾毅
Affiliation华北煤炭医学院生物科学系
中国疾病预防控制中心病毒病预防控制所肿瘤病毒室传染病预防控制国家重点实验室病毒性肿瘤组
郑州大学医学院
北京大学人民医院中心手术室
071002,河北大学生命科学院
Keywords食管肿瘤

乳头状瘤病毒,人
聚合酶链反应
Esophageal neoplasms
Carcinoma
Papillomavirus,human
Polymerase chain reaction
Issue Date2008
Publisher中华实验和临床病毒学杂志
Citation中华实验和临床病毒学杂志.2008,22,(4),251-253.
Abstract目的 探讨人乳头瘤病毒(human papillomavirus,HPV)与我国河南地区食管癌发生的相关性.方法 应用HPV L1通用引物GP5+/6+、HPV16E6和HPV18E6型特异性引物多聚酶链反应(Polymerase chain reaction,PCR),检测林州市食管癌组织中HPV存在状况.结果 31例食管癌组织中,29例检测到HPV阳性,阳性率为93.5%;其中19例检测到HPV16 E6基因,阳性率为61.3%,8例为HPV18 E6基因阳性,阳性率为25.8%,5例HPV16E6和18E6基因均阳性,为混合感染.HPV16和18型阳性率为71.0%.结论 我国河南省林州市食管癌组织中有HPV存在,并且HPV感染可能是食管癌发生的重要病因.
Objective To investigate the relationship between the human papillomavirus (HPV) and esophageal carcinomas. Methods We detected HPV DNA in 31 fresh tissue of esophageal carcinomas from Linzhou City, Henan Province, by PCR with the general primer set of GP5+/6+ for HPV L1 gene and type-specific primer sets for HPV16 and 18 as well. Results 29 from 31 esophageal carcinoma samples were HPV positive and the rate was 93.5%. Among the samples detected, 19 were HPV16E6 positive and rate was 61.3%,eight were HPV18 E6 positive and rate was 25.8% ; our result also showed five were the multiple infection containing HPV16 and 18 as well and the rate was 71.0%. Conclusion The high rate of HPV in the esophageal carcinoma samples suggested that HPV plays an etiologic role in the development of esophageal cancer in Linzhou City, Henan Province.
URIhttp://hdl.handle.net/20.500.11897/266863
ISSN1003-9279
Appears in Collections:未确定






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