Title过氧化物还原酶3在宫颈病变中的表达特点
Other TitlesExpression of peroxiredoxin Ⅲ in cervical lesions
Authors李连芹
陈春玲
曹泽毅
廖秦平
杜海军
詹少兵
周玲
曾毅
Affiliation清华大学第二附属医院妇产中心,北京,100049
北京大学第一医院妇产科
中国疾病预防控制中心病毒病预防控制所肿瘤室
Keywords过氧化物酶类
宫颈疾病
乳头状瘤病毒

依赖病毒
活性氧
Peroxides
Cervix diseases
Papillomavirus
human
Dependovixus
Reactive oxygen species
Issue Date2009
Publisher中华实验和临床病毒学杂志
Citation中华实验和临床病毒学杂志.2009,23,(6),443-445.
Abstract目的 检测过氧化物还原酶3在宫颈病变中的表达特点,以进一步探讨宫颈癌发生、发展的作用机制.方法 用免疫组织化学染色方法检测过氧化物还原酶3在宫颈癌中的表达特点.另外用含有人乳头瘤病毒16亚型E6/E7基因的重组腺相关病毒转染宫颈上皮细胞,通过实时定量PCR和蛋白质印迹法检测转染前后过氧化物还原酶3表达水平的变化.结果 过氧化物还原酶3在宫颈癌中的表达显著高于正常宫颈组织.宫颈上皮细胞感染重组腺相关病毒前后过氧化物还原酶3的表达水平没有显著变化.结论 过氧化物还原酶3与高危型HPV感染以及宫颈上皮细胞的恶性转化没有直接关系.而是在官颈癌发病以后,由于肿瘤细胞内的活性氧族增多,导致过氧化物还原酶3的表达上调.
Objective To investigate the expression feature of peroxiredoxin Ⅲ in cervical lesions and to further understand the mechanism for cervical cancer development/progression.Methods Expression of peroxiredoxin Ⅲ was immunohistochemically detected in cervical cancer.In addition.cervical epithelia were transfected with recombinant adeno-associated virus vector containing human papillomavirus 16 E6/E7 and pemxiredoxin Ⅲ expression was detected by quantitative real time PCR and Westem blotting.Results Peroxiredoxin Ⅲ was significantly up-regulated in cervical cancer tissues.Nevertheless,expression of peroxiredoxin Ⅲ remained unchanged in cervical epitllelial cells after transfection.Conclusion It seems that Prx Ⅲ is not related to cervical cancer initiation. Up-regulation of peroxiredoxin Ⅲ in cervical cancer might be an active response to oxidative stress in malignant cells,which protects against oxidatiton-induced apoptosis.
URIhttp://hdl.handle.net/20.500.11897/289000
ISSN1003-9279
Appears in Collections:未确定






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