Title保定地区食管癌患者癌组织中人乳头瘤病毒感染的检测
Other TitlesDetection of human papillomavirus in esophageal carcinoma tissues from Baoding City of Hebei Province
Authors赵晓瑜
李淑英
李颖
王晓莉
李玉兰
吴晓舟
周玲
刘宏图
曾毅
Affiliation河北大学生命科学院,071002
中国疾病预防控制中心病毒病预防控制所肿瘤病毒室病毒基因工程国家重点实验室
河北大学附属医院
北京大学人民医院中心手术室
Keywords食管肿瘤

乳头状瘤病毒,人
聚合酶链反应
Esophageal neoplasms
Carcinoma
Papillomavirus,human
Polymerase chain reaction
Issue Date2009
Publisher中华实验和临床病毒学杂志
Citation中华实验和临床病毒学杂志.2009,23,(2),91-93.
Abstract目的 探讨人乳头瘤病毒(human papillomavirus,HPV)与我国河北省保定地区食管癌发生的相关性.方法 应用HPV LI通用引物GP5+,6+、HPV16 E6和HPV18 E6型特异性引物多聚酶链反应(Polymerase chain reaction,PCR),检测保定地区食管癌组织中HPV存在状况.结果 42例食管癌组织中,37例检测到HPV阳性,阳性率为88.1%;其中19例检测到HPV16 E6基因,阳性率为45.2%,8例为HPV18 E6基因阳性,阳性率为19.0%,5例HPV16 E6和18 E6基因均阳性,为混合感染.结论 我国河北省保定地区食管癌组织中有HPV存在,并且HPV感染可能是食管癌发生的重要影响因子.
Objective To investigate the relationship between the human papillomavirus (HPV) and esophageal carcinoma in Baoding City of Hebei Province. Methods We detected HPV DNA in 42 formalin-fixed and paraffin-embedded tissues from surgically resected esophageal carcinomas from Baoding City of Hebei Province, by PCR with the general primer set of GP5 +/6 + for HPV L1 gene and type-specific primer sets for HPV16 and 18 as well. Results 37 from 42 esophageal carcinoma samples were HPV positive and the rate was 88.1% . Among the samples detected, 19 were HPV16 E6 positive and rate was 45.2%, eight were HPV18 E6 positive and rate was 19.0%. Conclusion The high rate of HPV in the esophageal carcinoma samples suggested that HPV plays an etiologic role in the development of esophageal cancer in Baoding City of Hebei Province.
URIhttp://hdl.handle.net/20.500.11897/289921
ISSN1003-9279
Appears in Collections:未确定






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