Title尘螨主要变应原DNA疫苗对其提取液诱导小鼠肺部变应性炎症的免疫保护作用
Other TitlesThe immunotherapeuftc effect of DNA vaccine of mite major allergen on mite extract triggered allergic pulmonary diseases
Authors郝敏麒
徐军
钟南山
Affiliation100034,北京大学第一医院呼吸科
广州呼吸疾病研究所
KeywordsDNA疫苗
变应原
免疫治疗
Issue Date2002
Publisher中华结核和呼吸杂志
Citation中华结核和呼吸杂志.2002,25,(3),159-161.
Abstract目的探讨尘螨主要变应原DNA疫苗对尘螨提取液诱导的小鼠肺部变应性炎症的免疫治疗作用.方法分别构建表达Derp1和Derp2的真核表达质粒pCMV-Derp 1和pDerp 2-IRES-Derp1.30只BALB/c小鼠随机分为正常组(6只)、对照组(12只)、单质粒组 (6只)和混合组(6只).后三组分别用空白质粒、pDerp2-IRES-Derp 1或混合的pDerp 2-IRES-Derp 1和pCMV-Derp1免疫,4周后用尘螨提取液致敏、激发,观察肺部炎症、计分、肺泡灌洗液(BALF)细胞总数和分类计数;酶联免疫吸附法(ELISA)检测BALF中白细胞介素4(IL-4)和γ干扰素(IFN-γ)水平.结果细胞总数和嗜酸细胞(EOS)计数:混合组[(6±4)×105/ml、0.05±0.04]和对照组[(21±13)×105/L、1.80±1.39]比较差异有显著性(P<0.05);IL-4水平:混合组[(168±233)g/L]与对照组[(538±256)g/L]比较差异有显著性(P<0.05),各组间IFN-γ比较差异无显著性(P>0.05).结论尘螨主要变应原的DNA疫苗可有效抑制尘螨提取液诱导的肺部变应性炎症,能表达两个主要变应原的DNA疫苗其抑制变应性炎症效果优于只表达一种变应原的疫苗.
To assess the effects of DNA vaccine of mite major allergens on murine pulmonary allergic inflammation triggered by mite extract.Two eukarycyte expression plasmids of Derp 1 and Derp 2 (pDerp 2-IRES-Derp 1 and pCMV-Derp 1) were constructed. BALB/c mice were divided into four groups: normal group, control group, mono-plasmid group and mixture immunization group. The latter three groups were immunized with blank plasmid, pDerp 2-IRES-Derp 1 and mixture of both pDerp 2-IRES-Derp 1 and pCMV-Derp 1, respectively. The mice were sensitized and challenged with mite extract 4 weeks later. Pathological scores, bronchoalveolar lavage (BAL) total cell count and differentiation were studied. IL-4 and IFN-gamma levels in BAL fluid (BALF) were detected by ELISA.Total cell count and eosinophil count in the mixture immunization group [(6 +/- 4) x 10(5)/ml], 0.05 +/- 0.04 were significantly lower than those in the control group [(21 +/- 13)10(5)/ml], 1.80 +/- 1.39, (P < 0.05). IL-4 in BALF in the mixture immunization [(168 +/- 233) g/L] group was significantly lower than that in the control group [(538 +/- 256) g/L], (P < 0.05). IFN-gamma showed no significant changes among different groups (P > 0.05).DNA vaccine of mite major allergens showed inhibitory effect on pulmonary allergic inflammation induced by mite extract, and this effect was more prominent in the DNA vaccine expressing two allergens than that expressing only one allergen.
URIhttp://hdl.handle.net/20.500.11897/121652
ISSN1001-0939
DOI10.3760/j:issn:1001-0939.2002.03.014
IndexedPubMed
中文核心期刊要目总览(PKU)
中国科学引文数据库(CSCD)
Appears in Collections:第一医院

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