Title | CD7和(或)CD56阳性急性髓系白血病患者干细胞表型分析及在微量残留病检测中的意义 |
Other Titles | The immunophenotypic analysis of CD7+ and(or)CD56+ acute myeloid leukemic stem cells and its applications in minimal residual disease detection |
Authors | 曹晖 王亚哲 吴红红 常艳 郝乐 陈珊珊 黄晓军 陆道培 刘艳荣 |
Affiliation | 北京大学人民医院,100044 |
Keywords | 白血病干细胞 白血病,非淋巴细胞,急性 免疫表型分析 微量残留病 |
Issue Date | 2008 |
Publisher | 中华血液学杂志 |
Citation | 中华血液学杂志.2008,29,(1),23-28. |
Abstract | 目的 探讨CD7和(或)CD56阳性急性髓系白血病(AML)干细胞免疫表型特点及微量残留病(MRD)与白血病干细胞LSC的关系.方法 采用四色流式细胞术,以4~6组四色抗体组合检测51例CD7+和(或)CD56+及CD34+CD38+初治AML患者(除外M3)白血病细胞免疫表型,并检测CD34+CD38-Lin-LSC中CD123、CD90、CD117、CD7和CD56的表达.以28名正常人骨髓作为对照,以CD34+CD38+细胞同时表达CD7和CD56作为白血病细胞标志,对26例CD7+和(或)CD56+AML患者的53份标本进行MRD监测.结果 CD7+和(或)CD56+初治AML患者CD34+CD38+细胞和CD34+CD38-Lin-干细胞中,CD7+细胞相对比例分别为(77.39&#177;20.71)%,(44.57&#177;22.70)%,CD56+细胞分别为(56.71&#177;32.56)%,(33.51&#177;29.64)%,均明显高于正常对照(P<0.01和P<0.05).CD34+CD38-Lin-干细胞中低表达CD90(P<0.01),高表达CD123(P<0.01);CD117的表达和正常对照相比差异无统计学意义(P>0.05).在随访的CD7+和(或)CD56+患者中,MRD阳性组CD34+CD38-Lin-亚群中CD7和(或)CD56表达的相对比例和实际比例增高的患者比例明显高于MRD阴性组,CD7+细胞实际比例增高的患者分别为71%(21例中15例)与16%(25例中4例)(P=0.001),相对比例增高的患者分别为81%(21例中17例)与24%(25例中6例)(P=0.000).CD56+细胞实际比例增高的患者分别为100%(4例)与12%(25例中3例)(P=0.001),相对比例增高的患者分别为75%(4例中3例)与20%(25例中5例)(P=0.031).CD7+AML患者中初治时CD7+CD34+CD38-Lin-细胞亚群实际比例高的患者治疗后较易出现MRD(P<0.05).结论 CD7和CD56在AML患者的异常表达发生在AML细胞分化的早期阶段,治疗后CD34+CD38-Lin-干细胞中CD7+和CD56+细胞比例高的患者易出现MRD. To explore the immunophenotypic characteristics of CD7 and/or CD56 positive acute myeloid leukemic stem cells, and the relationship between minimal residual disease (MRD) and the leukemic stem cells (LSC).The immunophenotype of leukemia cells from 51 CD34+ CD38+ CD7+ and/or CD34+ CD38+ CD56+ acute myeloid leukemia (AML) patients (exclude M3) at diagnosis was analyzed by using 4 - 6 panels of 4 color antibodies, and cells from 28 normal bone marrow (NBM) samples were served as control. The expression of CD7 and CD56 in the CD34+ CD38+ subpopulation was used as a leukemic cell marker for monitoring MRD of 53 samples from 26 CD7+ and (or) CD56+ patients.In CD7+ and/or CD56+ AML patients at diagnosis, the average positivity of CD7 in CD34+ CD38+ subpopulation and CD34+ CD38- Lin- stem cells subpopulation was (77.39 +/- 20.71)% and (44.57 +/- 22.70)%, and that of CD56 was (56.71 +/- 32.56)% and (33.51 +/- 29.64)%, respectively, all significantly higher than that of NBM (P < 0.01 and P < 0.05). Compared with that of NBM, the expression of CD90 in AML patients was significantly lower in the CD34+ CD38- Lin- subpopulation (P < 0.01), the expression of CD123 was significantly higher than NBM (P < 0.01), and the expression of CD117 was no significant difference (P > 0.05). In follow up of CD7+ and (or) CD56+ patients, the expression rate of CD7 and (or) CD56 in the CD34+ CD38- Lin- subpopulation MRD+ group was significantly higher than that in the MRD- group. The actual rate for CD7 was 71% (15/21) and 16% (4/25) (P = 0.001), and its relative rate was 81% (17/ 21) and 24% (6/25) (P = 0.000), respectively. The actual rate of CD56 is 100% (4/4) and 12% (3/25) (P = 0.001), and its relative rate was 75% (3/4) and 20% (5/25) (P = 0.031), respectively. A high CD7+ CD34+ CD38- Lin- subpopulation frequency at diagnosis in CD7+ AML patients predicted a high frequency of positive MRD in later detection (P < 0.05).CD7 and CD56 are expressed on the stem cells in CD7+ and/or CD56+ AMLs and a high frequency of CD7 and CD56 in the CD34+ CD38- Lin- stem cell subpopulation predicts a high frequency of positive MRD in later detection. |
URI | http://hdl.handle.net/20.500.11897/125122 |
ISSN | 0253-2727 |
DOI | 10.3321/j.issn:0253-2727.2008.01.006 |
Indexed | PubMed 中文核心期刊要目总览(PKU) 中国科技核心期刊(ISTIC) 中国科学引文数据库(CSCD) |
Appears in Collections: | 人民医院 |