Title间接免疫荧光法检测抗内皮细胞抗体在白塞病诊断中的意义
Other TitlesThe significance of anti-endothelial cell antibody AECA in Behcet's disease
Authors贾汝琳
李茹
李永哲
卢靓
贾园
栗占国
Affiliation北京大学人民医院风湿免疫科,100044
中国医学科学院,北京协和医科大学,北京协和医院检验科
Keywords贝赫切特综合征
荧光抗体技术,间接
抗内皮细胞抗体
Behcet&#39
s syndrome
Fluorescent antibody technique,indirect
Anti-endothelial cellantibody
Issue Date2008
Publisher中华风湿病学杂志
Citation中华风湿病学杂志.2008,12,(10),688-690,插2.
Abstract目的 探讨抗内皮细胞抗体(AECA)在白塞病(BD)诊断和鉴别诊断中的意义,并分析该抗体与BD患者临床特点及与其他免疫学指标的相关性.方法 采用间接免疫荧光法(IIF),以人脐静脉内皮细胞为底物抗原,检测73例白塞病患者、60例系统性红斑狼疮(SLE)患者、35例系统性硬化症(SSc)患者、30例类风湿关节炎(RA)患者、62例系统性血管炎患者和40名健康献血员血清中的AECA,并评价其与BD患者临床指标的关系.结果 ①AECA在BD患者中的敏感性为54.8%,特异性为87.2%.与SLE患者(8%)、SSc患者(9%)、RA患者(10%)、系统性血管炎患者(27%)及正常对照(2%)相比,BD患者血清AECA的阳性率(54.8%)明显增高(均P<0.01).②在BD患者中,ANA及其他常见自身抗体、低补体血症、蛋白尿等发生率分别为(5.5%、1.4%、11.O%、6.8%),明显低于疾病对照组(p<0.01). ③AECA阳性BD患者红细胞沉降率明显高于AECA阴性患者(P<0.01).结论 AECA在BD中有较高的敏感性和特异性,可作为诊断该病的血清学指标之一.
Objective To evaluate the significance of anti-endothelial cell antibody (AECA) and the correlation between AECA and clinical features of Behcet's disease (BD). Methods AECA was detected in 73 BD, 60 SLE, 35 SSc, 30 RA, 62 systemic vasculitis patients and 40 health controls by indirect immunofluorescence analysis in which the endothelial cells from umbilical vein were used as the substrate. Results Higher frequency of AECA was found in BD (54.8%) with the specificity of 87.2%, compared to those in SLE (8%), SSc (9%), RA (10%), systemic vasculitis (27%) and healthy controls (2%, P<O.O1, respectively). However, the prevalence of ANA, other common auto-antibodies, hypocomplementemia, throm- bopenia and proteinuria were significantly lower in BD when compared with those with other autoimmune diseases (P<0.01). In addition, AECA- positive BD patients had significantly higher ESR than those with negative AECA (P<0.01). Conclusion AECA is one of the major parameters for the diagnosis of BD with high sensitivity and specificity.
URIhttp://hdl.handle.net/20.500.11897/132846
ISSN1007-7480
DOI10.3321/j.issn:1007-7480.2008.10.010
Indexed中文核心期刊要目总览(PKU)
中国科技核心期刊(ISTIC)
中国科学引文数据库(CSCD)
Appears in Collections:人民医院

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