TitleVanadyl acetylacetonate upregulates PPAR gamma and adiponectin expression in differentiated rat adipocytes
AuthorsWu, Yaling
Huang, Meiling
Zhao, Pan
Yang, Xiaoda
AffiliationPeking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Labs Nat & Biomimet Drugs, Beijing 100191, Peoples R China.
Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China.
KeywordsVanadium
Adiponectin
Adipocytes
Peroxisome-proliferator-activated receptor gamma
p38 mitogen-activated protein kinase
GENE-EXPRESSION
SKELETAL-MUSCLE
OB/OB MICE
IN-VITRO
INSULIN
PROTEIN
COMPLEX
MULTIMERIZATION
MECHANISMS
RECEPTORS
Issue Date2013
Publisherjournal of biological inorganic chemistry
CitationJOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY.2013,18,(6),623-631.
AbstractVanadium compounds are promising agents in the therapeutic treatment of diabetes mellitus, but their mechanism of action has not been fully elucidated. The current work investigated the effects of vanadyl acetylacetonate, VO(acac)(2), on peroxisome-proliferator-activated receptor gamma (PPAR gamma) and adiponectin, which are important targets of antidiabetic drugs. The experimental results revealed that vanadyl complexes increased the expression and multimerization of adiponectin in differentiated rat adipocytes. VO(acac)(2) caused activation of p38 mitogen-activated protein kinase (MAPK) and AMP-activated protein kinase (AMPK) and elevation of PPAR gamma levels. The specific inhibitors SB203580 (p38 MAPK inhibitor) and T0070907 (PPAR gamma inhibitor) decreased the expression of adiponectin; however, compound C (AMPK inhibitor) did not significantly reduce the expression of adiponectin. In addition, vanadyl complexes induced protein-protein interaction between PPAR gamma and a vanadium-binding chaperone, heat shock protein 60 kDa. Overall, our results suggest that vanadyl complexes may upregulate PPAR gamma by suppressing PPAR gamma degradation, and thus stimulate adiponectin expression and multimerization. The present work has provided new insights into the mechanism of the antidiabetic actions of vanadium compounds.
URIhttp://hdl.handle.net/20.500.11897/156425
ISSN0949-8257
DOI10.1007/s00775-013-1007-3
IndexedSCI(E)
PubMed
Appears in Collections:天然药物与仿生药物国家重点实验室

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