| Title | 血浆纤溶酶原激活物抑制物1与胰岛素抵抗的相关性研究 |
| Other Titles | The relationship between PAI-1 and insulin resistance syndrome |
| Authors | 董砚虎 王海燕 司元国 吕文山 王军 柳林 钱荣立 |
| Affiliation | 266033,青岛市内分泌糖尿病研究所 北京大学第一医院内分泌科 |
| Keywords | 胰岛素抵抗 基因 血浆纤溶酶原激活物抑制物1 Insulin resistance syndrome |
| Issue Date | 2002 |
| Publisher | 中国糖尿病杂志 |
| Citation | 中国糖尿病杂志.2002,10,(4),222-226. |
| Abstract | 目的探讨血浆纤溶酶原激活物抑制物1(plasminogen activitor inhibitor one,PAI-1)活性及其基因启动子区4G/5G多态性与胰岛素抵抗综合征的关系. 方法应用等位基因特异性PCR扩增技术,对胰岛素抵抗综合征患者(160例)和对照组(90例)PAI-1 4G/5G多态位点的基因型进行检测,用发色底物法测血浆PAI-1活性. 结果 (1)胰岛素抵抗综合征患者PAI-1活性明显升高,空腹胰岛素、空腹血糖、甘油三酯与PAI-1活性升高密切相关(P<0.01).(2)对照组和胰岛素抵抗综合征患者,4G/5G基因型频率分别为0.52和0.48,两组比较无明显差异(P>0.05),但胰岛素抵抗综合征不同基因型者PAI-1活性差异显著(P<0.01),4G/4G基因型者PAI-1活性高于4G/5G和5G/5G基因型者(P<0.01).(3)血糖、甘油三酯对PAI-1活性的调节受基因型的影响.4G/4G基因型者血糖、甘油三酯与PAI-1活性密切相关(P<0.01),而4G/5G和5G/5G基因型者血糖、甘油三酯与PAI-1活性无明显相关(P>0.05).(4)胰岛素对PAI-1活性的影响无基因型依赖性. 结论 PAI-1活性升高是胰岛素抵抗综合征患者的特征之一,免疫反应性胰岛素、血糖、甘油三酯与PAI-1活性升高有关,血糖、甘油三酯对PAI-1活性的调节存在明显的基因型依赖性. Objective To investigate the corelationship between PAI 1 activity and PAI 1 promoter 4G/5G polymorphism with insulin resistance syndrome. Methods The 4G/5G allele polymorphism in the PAI 1 gene promoter region were genotyped with an allele specific PCR in patients with insulin resistance syndrome (160)and controls (90).The activity of PAI 1 was assayed by chromogenic substrate method. Results (1)PAI 1 activity was higher in patients with insulin resistance syndrome than in controls.Insulin,blood glucose and triglyceride were responsible for it. (2)Though the frequency of PAI 1 4G/4G genotype was equal in two groups,the plasma PAI 1 activity was significantly different in different genotypes.It was higher in 4G/4G group than in 4G/5G( P <0.01) and 5G/5G group( P <0.01). (3)The level of PAI 1 activity regulated by blood glucose and triglyceride depended on PAI 1 4G/5G genotype. The levels of blood glucose and triglyceride were significantly associated with PAI 1 activity only in 4G/4G group. (4)The plasma PAI 1 activity was regulated by insulin in each group. Conclusion Increased PAI 1 activity caused by insulin ,blood glucose and triglyceride is a character of insulin resistance syndrome. The presence of the 4G allele of PAI 1 gene and higher blood glucose and triglyceride concentrations are associated with higher level of PAI 1 activity and may be involved in the generation of insulin resistance syndrome. |
| URI | http://hdl.handle.net/20.500.11897/227160 |
| ISSN | 1006-6187 |
| DOI | 10.3321/j.issn:1006-6187.2002.04.009 |
| Indexed | 中国科学引文数据库(CSCD) |
| Appears in Collections: | 第一医院 |
