TitleControlling DNA Translocation through Gate Modulation of Nanopore Wall Surface Charges
AuthorsHe, Yuhui
Tsutsui, Makusu
Fan, Chun
Taniguchi, Masateru
Kawai, Tomoji
AffiliationOsaka Univ, Inst Sci & Ind Res, Osaka 5670047, Japan.
Peking Univ, Ctr Comp, Beijing 100871, Peoples R China.
Osaka Univ, Inst Sci & Ind Res, 8-1 Mihogaoka, Osaka 5670047, Japan.
Keywordsnanopore sequencing
wall surface charges
gate control
SOLID-STATE NANOPORES
ATOMIC LAYER DEPOSITION
POLYNUCLEOTIDE MOLECULES
SINGLE
TRANSPORT
DISCRIMINATION
NUCLEOTIDES
SENSORS
FORCE
ACID
Issue Date2011
Publisheracs nano
CitationACS NANO.2011,5,(7),5509-5518.
AbstractOne major challenge of nanopore-based DNA sequencing technology is to find an efficient way to reduce DNA translocation speed so that each nucleotide can reside long enough in the pore for interrogation. Here we report the electrical tuning of DNA translocation speed by gate modulation of nanopore wall surface charges. We find that native surface-charge-induced counterions in the electroosmotic layer substantially enhance advection flow of fluid, which exerts stronger dragging forces on the translocating DNA, and thereby lowering the DNA translocation speed. We propose a feedback device architecture to regulate DNA translocation by modulating the effective wall surface charge density sigma(w)* via lateral gate voltages-at the beginning, a positive gate bias is applied to weaken sigma(w)* in order to enhance the capture rate of DNA molecule; upon detection of ionic current variance indicating DNA has been driven into the nanopore, gate bias is turned to be negative soffiat sigma(w)* is reinforced and DNA translocation is retarded. We show that a gate electric field can dramatically decrease the DNA translocation speed at a rate about 55 mu m/s per 1 mV/nm.
URIhttp://hdl.handle.net/20.500.11897/238939
ISSN1936-0851
DOI10.1021/nn201883b
IndexedSCI(E)
EI
Appears in Collections:计算中心

Web of Science®



Checked on Last Week

Scopus®



Checked on Current Time

百度学术™



Checked on Current Time

Google Scholar™





License: See PKU IR operational policies.