| Title | Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease |
| Authors | Wu, Tongbo Xiao, Xianjin Gu, Feidan Zhao, Meiping |
| Affiliation | Peking Univ, Coll Chem & Mol Engn, MOE Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China. |
| Keywords | SINGLE-NUCLEOTIDE POLYMORPHISMS SWITCH-ON PROBE MYELOPROLIFERATIVE DISORDERS NUCLEIC-ACIDS GENOMIC DNA JAK2 V617F PCR OLIGONUCLEOTIDE AMPLIFICATION MUTATIONS |
| Issue Date | 2015 |
| Publisher | CHEMICAL COMMUNICATIONS |
| Citation | CHEMICAL COMMUNICATIONS.2015,51,(98),17402-17405. |
| Abstract | An abasic site modified fluorescent probe has been developed which enabled the rapid discrimination of stable single mismatched base pairs by lambda exonuclease with remarkably high discrimination factors (447 for T:G and 238 for A:G). This method allowed sensitive detection of single nucleotide variation at very low abundances (0.02-0.05% mutant-to-wild type). |
| URI | http://hdl.handle.net/20.500.11897/417506 |
| ISSN | 1359-7345 |
| DOI | 10.1039/c5cc05749c |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 化学与分子工程学院 生物有机与分子工程教育部重点实验室 |
