|Title||F-127-PEI co-delivering docetaxel and TFPI-2 plasmid for nasopharyngeal cancer therapy|
|Affiliation||Southern Med Univ, Zhujiang Hosp, Dept Otolaryngol, Guangzhou 510282, Guangdong, Peoples R China.|
Jiangxi Prov Peoples Hosp, Jiangxi Key Lab Hematol Oncol & Cell Biol, Nanchang 330006, Peoples R China.
Zhejiang Sci Tech Univ, Sch Life Sci, Hangzhou 310018, Zhejiang, Peoples R China.
Jiangxi Inst Mat Med, Dept Pharmacol, Nanchang 330029, Peoples R China.
Guangdong Polytech, Dept Light Chem Engn, Foshan 528041, Peoples R China.
Peking Univ, Shenzhen Hosp, Dept Otolaryngol, Shenzhen 518036, Peoples R China.
Southern Med Univ, Zhujiang Hosp, Dept Otolaryngol, Guangzhou 510282, Guangdong, Peoples R China.
Nie, GH (reprint author), Peking Univ, Shenzhen Hosp, Dept Otolaryngol, Shenzhen 518036, Peoples R China.
|Publisher||MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS|
|Citation||MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS.2016,61,269-277.|
|Abstract||The co-delivery of drug and gene has become the primary strategy in cancer therapy. However, to construct one safe co-delivering system with higher drug loading and gene transfection efficiency for cancer therapy is still challenging. Herein, a novel degradable nanocarriers were synthesized and characterized in this study, which was composed of polyethylenimine (PEI)-linked PEO-PPO-PEO (Pluronic F127), called F127-PEI. Then the nanocarrier was used for hydrophobic docetaxel (DOC) and functional gene (TFPI-2 plasmid) co-delivery to treat nasopharyngeal cancer (NPC). The results indicated that F127-PEI nanocarriers had higher DOC loading amount and possessed good gene delivery effect in vitro. For co-delivery analysis, the obtained F127-PEI/DOC/TFPI-2 complexes could induce a more significant apoptosis than DOC or TFPI-2 alone, and decreased invasive capacity of NPC HNE-1 cells more obviously. Moreover, the F127-PEI copolymer exhibited better blood compatibility and lower cytotoxicity compared to PEI-25k by the hemolysis and MTT assays, which suggests a promising potential for NPC therapy. (C) 2015 Elsevier B.V. All rights reserved.|
|Appears in Collections:||深圳医院|