TitleComparison of commonly used retrograde tracers in rat spinal motor neurons
AuthorsYu, You-lai
Li, Hai-yan
Zhang, Pei-xun
Yin, Xiao-feng
Han, Na
Kou, Yu-hui
Jiang, Bao-guo
AffiliationPeking Univ, Peoples Hosp, Dept Trauma & Orthoped, Beijing 100871, Peoples R China.
Weifang Peoples Hosp, Dept Gastroenterol, Weifang, Shandong, Peoples R China.
Keywordsnerve regeneration
tracing efficacy
fluorescent tracers
retrograde tracing
femoral nerve
motor neurons
PERIPHERAL-NERVE INJURY
FLUORESCENT TRACERS
FAST-BLUE
AFFERENT NEURONS
FEMORAL NERVE
FLUORO-RUBY
REINNERVATION
PATHWAYS
REGENERATION
ACCURACY
Issue Date2015
PublisherNeural Regeneration Research
CitationNeural Regeneration Research.2015,10,(10),1700-1705.
AbstractThe purpose of this study was to investigate the effect of four fluorescent dyes, True Blue (TB), Fluoro-Gold (FG), Fluoro-Ruby (FR), and 1,1'-dioctadecy1-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI), in retrograde tracing of rat spinal motor neurons. We transected the muscle branch of the rat femoral nerve and applied each tracer to the proximal stump in single labeling experiments, or combinations of tracers (FG-Dif and TB-DiI) in double labeling experiments. In the single labeling experiments, significantly fewer labeled motor neurons were observed after FR labeling than after TB, FG, or DiI, 3 days after tracer application. By 1 week, there were no significant differences in the number of labeled neurons between the four groups. In the double-labeling experiment, the number of double-labeled neurons in the FG-DiI group was not significantly different from that in the TB-Dif group 1 week after tracer application. Our findings indicate that TB, FG, and DiI have similar labeling efficacies in the retrograde labeling of spinal motor neurons in the rat femoral nerve when used alone. Furthermore, combinations of Dif and TB or PG are similarly effective. Therefore, of the dyes studied, TB, PG and DiI, and combinations of DiI with TB or FG, are the most suitable for retrograde labeling studies of motor neurons in the rat femoral nerve.
URIhttp://hdl.handle.net/20.500.11897/451442
ISSN1673-5374
DOI10.4103/1673-5374.167772
IndexedSCI(E)
PubMed
中国科技核心期刊(ISTIC)
中国科学引文数据库(CSCD)
Appears in Collections:人民医院

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