TitleEstrogen receptors in granulosa cells govern meiotic resumption of pre-ovulatory oocytes in mammals
AuthorsLiu, Wei
Xin, Qiliang
Wang, Xiao
Wang, Sheng
Wang, Huarong
Zhang, Wenqiang
Yang, Ye
Zhang, Yanhao
Zhang, Zhiyuan
Wang, Chao
Xu, Yang
Duan, Enkui
Xia, Guoliang
AffiliationChina Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, No 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China.
Peking Univ, Hosp 1, Med Ctr Reprod & Genet, Dept Gynecol & Obstet, Beijing 100034, Peoples R China.
Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China.
Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China.
KeywordsFOLLICLE-STIMULATING-HORMONE
NPR2 GUANYLYL CYCLASE
IN-VITRO
NATRIURETIC PEPTIDE
OVARIAN-FOLLICLE
MOUSE OVARY
CYCLIC-GMP
RODENT OOCYTES
SOMATIC-CELLS
PROPHASE I
Issue Date2017
PublisherCELL DEATH & DISEASE
CitationCELL DEATH & DISEASE.2017,8.
AbstractIn mammals, oocytes are arrested at the diplotene stage of meiosis I until the pre-ovulatory luteinizing hormone (LH) surge triggers meiotic resumption through the signals in follicular granulosa cells. In this study, we show that the estradiol (E2)-estrogen receptors (ERs) system in follicular granulosa cells has a dominant role in controlling oocyte meiotic resumption in mammals. We found that the expression of ERs was controlled by gonadotropins under physiological conditions. E2-ERs system was functional in maintaining oocyte meiotic arrest by regulating the expression of natriuretic peptide C and natriuretic peptide receptor 2 (NPPC/NPR2), which was achieved through binding to the promoter regions of Nppc and Npr2 genes directly. In ER knockout mice, meiotic arrest was not sustained by E2 in most cumulus-oocyte complexes in vitro and meiosis resumed precociously in pre-ovulatory follicles in vivo. In human granulosa cells, similar conclusions are reached that ER levels were controlled by gonadotropins and E2-ERs regulated the expression of NPPC/NPR2 levels. In addition, our results revealed that the different regulating patterns of follicle-stimulating hormone and LH on ER levels in vivo versus in vitro determined their distinct actions on oocyte maturation. Taken together, these findings suggest a critical role of E2-ERs system during oocyte meiotic progression and may propose a novel approach for oocyte in vitro maturation treatment in clinical practice.
URIhttp://hdl.handle.net/20.500.11897/474831
ISSN2041-4889
DOI10.1038/cddis.2017.82
IndexedSCI(E)
Appears in Collections:第一医院

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