TitleCell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells
AuthorsVeevers, Jennifer
Farah, Elie N.
Corselli, Mirko
Witty, Alec D.
Palomares, Karina
Vidal, Jason G.
Emre, Nil
Carson, Christian T.
Ouyang, Kunfu
Liu, Canzhao
van Vliet, Patrick
Zhu, Maggie
Hegarty, Jeffrey M.
Deacon, Dekker C.
Grinstein, Jonathan D.
Dirschinger, Ralf J.
Frazer, Kelly A.
Adler, Eric D.
Knowlton, Kirk U.
Chi, Neil C.
Martin, Jody C.
Chen, Ju
Evans, Sylvia M.
AffiliationUniv Calif San Diego, Sch Med, 9500 Gilman Dr, La Jolla, CA 92093 USA.
BD Biosci, 11077 North Torrey Pines Rd, La Jolla, CA 92037 USA.
Peking Univ Shenzhen, Grad Sch, Key Lab Chem Gen, Drug Discovery Ctr, Shenzhen 518055, Peoples R China.
Univ Calif San Diego, Skaggs Sch Pharm, 9500 Gilman Dr, La Jolla, CA 92093 USA.
Univ Calif San Diego, Dept Pediat, 9500 Gilman Dr, La Jolla, CA 92093 USA.
Univ Calif San Diego, Inst Genom Med, 9500 Gilman Dr, La Jolla, CA 92093 USA.
Univ Calif San Diego, Sch Med, 9500 Gilman Dr, La Jolla, CA 92093 USA.
Evans, SM (reprint author), Univ Calif San Diego, Skaggs Sch Pharm, 9500 Gilman Dr, La Jolla, CA 92093 USA.
Keywordscardiac differentiation,cell-surface marker signature,human embryonic stem cells,ventricular cardiomyocytes
CARDIAC DIFFERENTIATION
GENERATION
SELECTION
MYOCYTES
DEVELOP
ANTIGEN
MOUSE
Issue Date2018
PublisherSTEM CELL REPORTS
CitationSTEM CELL REPORTS. 2018, 11(3), 828-841.
AbstractTo facilitate understanding of human cardiomyocyte (CM) subtype specification, and the study of ventricular CM biology in particular, we developed a broadly applicable strategy for enrichment of ventricular cardiomyocytes (VCMs) derived from human embryonic stem cells (hESCs). A bacterial artificial chromosome transgenic H9 hESC line in which GFP expression was driven by the human ventricular-specific myosin light chain 2 (MYL2) promoter was generated, and screened to identify cell-surface markers specific for MYL2-GFP-expressing VCMs. A CD77(+)/CD200(-) cell-surface signature facilitated isolation of >97% cardiac troponin I-positive cells from H9 hESC differentiation cultures, with 65% expressing MYL2-GFP. This study provides a tool for VCM enrichment when using some, but not all, human pluripotent stem cell lines. Tools generated in this study can be utilized toward understanding CM subtype specification, and enriching for VCMs for therapeutic applications.
URIhttp://hdl.handle.net/20.500.11897/517352
ISSN2213-6711
DOI10.1016/j.stemcr.2018.07.007
IndexedSCI(E)
PubMed
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