TitleBroadband graphene-based photoacoustic microscopy with high sensitivity
AuthorsYang, Fan
Song, Wei
Zhang, Chonglei
Min, Changjun
Fang, Hui
Du, Luping
Wu, Peng
Zheng, Wei
Li, Changhui
Zhu, Siwei
Yuan, Xiaocong
AffiliationShenzhen Univ, Nanophoton Res Ctr, Shenzhen 518060, Peoples R China.
Chinese Acad Sci, Res Lab Biomed Opt & Mol Imaging, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China.
Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China.
Tianjin Union Med Ctr, Inst Oncol, Tianjin 300121, Peoples R China.
KeywordsOPTICAL COHERENCE TOMOGRAPHY
FLUORESCENCE MICROSCOPY
ULTRASONIC DETECTOR
RESOLUTION
REFLECTION
BIOPSY
TISSUE
SENSOR
OXYGEN
Issue Date2018
PublisherNANOSCALE
CitationNANOSCALE. 2018, 10(18), 8606-8614.
AbstractPhotoacoustic microscopy (PAM) enables the measurement of properties associated with optical absorption within tissues and complements sophisticated technologies employing optical microscopy. An inadequate frequency response as determined by a piezoelectric ultrasonic transducer results, however, in poor depth resolution and inaccurate measurements of the coefficients of optical absorption. We developed a PAM system configured as an attenuated total reflectance sensor with a ten-layer graphene film sandwiched between a prism and water (the coupling medium) for photoacoustic (PA) wave detection. Transients of the PA pressure cause perturbations in the refractive index of the water thereby changing the polarization-dependent absorption of the graphene film. The signal in PA detection involves recording the difference in the temporal-varying reflectance intensity between the two orthogonally polarized probe beams. The graphene-based sensor has an estimated noise-equivalent-pressure sensitivity of similar to 550 Pa over an approximately linear pressure response from 11.0 kPa to 55.0 kPa. Moreover, it enables a much broader PA bandwidth detection of up to similar to 150 MHz, primarily dominated by a highly localized evanescent field. From the strong optical absorption of inherent hemoglobin, in vivo label-free PAM imaging provided a three-dimensional viewing of the microvasculature of a mouse ear. These results suggest great potential for graphene-based PAM in biomedical investigations, such as microcirculation studies.
URIhttp://hdl.handle.net/20.500.11897/524108
ISSN2040-3364
DOI10.1039/c7nr09319e
IndexedSCI(E)
EI
Appears in Collections:工学院

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