TitleBlinking Acoustic Nanodroplets Enable Fast Super-resolution Ultrasound Imaging
AuthorsDong, Feihong
An, Jian
Zhang, Jiabin
Yin, Jingyi
Guo, Wenyu
Wang, Di
Feng, Feng
Huang, Shuo
Zhang, Jue
Cheng, Heping
AffiliationPeking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
Peking Univ, Coll Future Technol, Natl Biomed Imaging Ctr,Inst Mol Med, Peking Tsinghua Ctr Life Sci,State Key Lab Membra, Beijing 100871, Peoples R China
Peking Univ, Natl Biomed Imaging Ctr, Beijing 100871, Peoples R China
Chinese Acad Med Sci, PKU Nanjing Inst Translat Med, Res Unit Mitochondria Brain Dis, Nanjing 211899, Peoples R China
Issue Date26-Oct-2021
PublisherACS NANO
AbstractThe advent of localization-based super-resolution ultrasound (SRUS) imaging creates a vista for precision vasculature and hemodynamic measurements in brain science, cardiovascular diseases, and cancer. As blinking fluorophores are crucial to super-resolution optical imaging, blinking acoustic contrast agents enabling ultrasound localization microscopy have been highly sought, but only with limited success. Here we report on the discovery and characterization of a type of blinking acoustic nanodroplets (BANDs) ideal for SRUS. BANDs of 200-500 nm diameters comprise a perfluorocarbon-filled core and a shell of DSPC, Pluronic F68, and DSPE-PEG2000. When driven by clinically safe acoustic pulses (MI < 1.9) provided by a diagnostic ultrasound transducer, BANDs underwent reversible vaporization and reliquefaction, manifesting as "blinks", at rates of up to 5 kHz. By sparse activation of perfluorohexane-filled BANDs-C-6 at high concentrations, only 100 frames of ultrasound imaging were sufficient to reconstruct super-resolution images of a no-flow tube through either cumulative localization or temporal radiality autocorrelation. Furthermore, the use of high-density BANDs-C6-4 (1 x 10(8)/mL) with a 1:9 admixture of perfluorohexane and perfluorobutane supported the fast SRUS imaging of muscle vasculature in live animals, at 64 mu m resolution requiring only 100 frames per layer. We anticipate that the BANDs developed here will greatly boost the application of SRUS in both basic science and clinical settings.
Appears in Collections:前沿交叉学科研究院

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