Title时间分辨荧光免疫分析法直接测定雌二醇
Other TitlesTime-resolved Fluorescence Immunoassay of Estradiol in Serum
Authors唐棣
王永成
常文保
慈云祥
郭振泉
Affiliation北京大学化学与分子工程学院,北京,100871
北京大学生命科学学院
Keywords雌二醇
单克隆抗体
洗脱增强-时间分辨荧光免疫分析法
estradiol
monoclonal antibody
time-resolved fluorescence immunoassay
Issue Date1999
Publisher分析化学
Citation分析化学.1999,27,(8),899-903.
Abstract合成了雌二醇抗原,并获得了雌二醇单克隆抗体,通过酶联免疫吸附法测得效价为1.0×107.使用洗脱增强-时间分辨荧光免疫分析法测定了血清中的雌二醇,浓度范围为1.0×10-2~1.0×103 μg/L,最低检出限为5.6 ng/L,批内RSD%小于3.7%,批间RSD%小于7.5%.将测定结果与放射免疫分析法作了比较.
A microtitre plate time-resolved fluorescence immunoassay (TRFIA) for the analysis of serum estradiol is described, involving competition between sample estradiol and an immobilized estradiol-bovine serum albumin sample for a monoclonal anti-estradiol antibody. The assay based on the qualification of the europium chelator, (chlorosulfonyl) thenoyltrifluoroacetone( CTTA) in the presence of excess Eu3+ in solution. The assay dose-response curve covered a ranger of 1 x 10(-5) similar to 1 x 10(-12) g/mL. The intra-and inter-assay coefficient of variation for the assay ranged from 0.6% to 3.7% and from 1.9% to 7.5% respectively. The assay showed satisfactory correlation with a standard estradiol radioimmunoassay.
URIhttp://hdl.handle.net/20.500.11897/78574
ISSN0253-3820
DOI10.3321/j.issn:0253-3820.1999.08.007
IndexedSCI(E)
中国科学引文数据库(CSCD)
Appears in Collections:化学与分子工程学院
生命科学学院

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