TitleDTPA-Eu~(3+)螯合物在核酸杂交分析中的应用
Other TitlesTHE APPLICATION OF DTPA-EU3+ COMPLEX TO DNA HYBRIDIZATION ASSAY
Authors慈云祥
陈勇
李元宗
常文保
Affiliation北京大学化学系
Keywords杂交分析
DTPA-Eu~(3+)
时间分辨荧光光度法
HYBRIDIZATION ASSAY
DTPA-EU3+
TIME RESOLVED FLUOROMETRY
Issue Date1995
Publisher高等学校化学学报
Citation高等学校化学学报.1995,(02),184-187.
Abstract报道了靶DNA/生物素化λDNA探针/亲合素/Bio-BSA(TG)-DTPA-Eu~(3+)体系在核酸杂交分析中的应用。亲合素做为连结杂交体与Bio-BSA-DTPA-Eu~(3+)或Bio-TG-DTPA-Eu~(3+)的桥,生物素和DTPA-Eu~(3+)均标记在载体蛋白(BSA,TG)上。优化了分析条件,比较了己二胺、乙二胺转胺化探针及载体蛋白对杂交分析的影响。方法灵敏度高(1pg/well靶λDNA),重现性较好(RSD=5.7%,n=6)。
Here we reports a new detection method for lambda DNA through the system of target DNA/Bio-DNA probe/Avidin/Bio-BSA (TG)-DTPA-Eu3+. Avidin was used as a bridge to link hybrids and Bio-BSA-DTPA-Eu3+ or Bio-TG-DTPA-Eu3+. The carrier proteins were labeled with both biotin and DTPA-Eu3+. The analytical conditions were optimized, The influences of transamination of DNA probe by ethylene diamine and 1,6-hexane diamine as well as carrier proteins on hybridization assay were compared, The method has a high sensitivity (1 pg/well) with good reproducibility (RSD=5.7%, n=6).
URIhttp://hdl.handle.net/20.500.11897/81153
ISSN02510790
IndexedSCI(E)
中文核心期刊要目总览(PKU)
中国科学引文数据库(CSCD)
Appears in Collections:化学与分子工程学院

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